Chlorsulfuron affects the biosynthesis of branched chain amino acids by inhibiting the enzyme acetolactate synthase (ALS). The crs1-1 gene from Arabi-dopsis thaliana confers resistance to chlorsulfuron (CS) by encoding an ALSS with a reduced affinity to Chlor-sulfuron. Chlorsulfuron has been applied as a succesful selective agent in the trans-formation of tobacco, maize and sugar-beet. Transgenic poplars and fertile rice plants have also been obtained by using the crs1-1 gene in combi-nation with the CaMV 35S promoter.
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