C0203

CHU (N6) Medium

Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.

Micro Elements mg/l µM
FeNaEDTA 36.70 100.00
H3BO3 1.60 25.88
KI 0.80 4.81
MnSO4.H2O 3.33 19.70
ZnSO4.7H2O 1.50 5.22
Macro Elements mg/l mM
CaCl2 125.33 1.13
KH2PO4 400.00 2.94
KNO3 2830.00 27.99
MgSO4 90.27 0.75
(NH4)2SO4 463.00 3.50

Chu C.C, The N6 medium and its application to anther culture of -cereal crops, Proc. Symp. Plant Tissue Cult., Peking, 43 (1978).Chu C.C. et al., Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen -sources. Scienta Sinic., 18, 659 (1975).

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water
Filename Language File size
C0203_GB.pdf English 154 KB
C0203_NL.pdf Nederlands 157 KB
C0203_DE.pdf Deutsch 160 KB
C0203_FR.pdf Français 160 KB
C0203_ES.pdf Español 159 KB
C0203_IT.pdf Italiano 157 KB
Filename Batch number Expiry date Language
C0203_P15035_GB.pdf P15035 07-2020 English
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