C0229

CLC / Ipomoea EP medium

Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM.

Micro Elements mg/l µM
CoCl2.6H2O 0.025
CuSO4.5H2O 0.025
FeNaEDTA 36.70
H3BO3 6.20
KI 0.83
MnSO4.H20 16.90
Na2MoO4.2H2O 0.25
ZnSO4.7H2O 8.60
Macro Elements mg/l mM
CaCl2 332.02
KH2PO4 170.00
KNO3 1900.00
MgSO4 180.54
NH4NO3 800.40
Vitamins mg/l µM
myo-Inositol 90.10
Nicotinic acid 1.23
Pyridoxine HCl 1.03
Thiamine HCl 1.69

Total concentration Micro and Macro elements (EP) including -vitamins: 3546.54 mg/l

Cheé R. et al., Optimizing Embryogenic Callus and Embryo Growth of a synthetic seed system for Sweetpotato by varying media nutrient concentrations. J. Am. Soc. Hort. Sci. 117, 663 (1992).

Filename Language File size
C0229_GB.pdf English 152 KB
C0229_NL.pdf Nederlands 153 KB
C0229_DE.pdf Deutsch 154 KB
C0229_FR.pdf Français 152 KB
C0229_ES.pdf Español 152 KB
C0229_IT.pdf Italiano 151 KB
  • C0229.0001
  • 1 l
  • € 2.20
  • Order
  • C0229.0010
  • 10 l
  • € 8.50
  • Order
  • C0229.0050
  • 50 l
  • € 26.50
  • Order
* All prices are ex-works Haarlem NL. Salestax, local prices and availability may vary in your region/country. Please contact our sales department or read our terms of sales.