C0248

CHÉE & POOL medium

In the medium defined by Chée and Pool the original Murashige and Skoog concentration of Chlorine, Iodine and Manganese is decreased, resulting in an improved shoot multiplication of Vitis. Substituting calcium nitrate for calcium chloride improved the quality of grapevine shoots produced in culture. Shoot multiplication was dramatically improved by ommitting Iodine and lowering the concen-tration of Manganese. This might be the result of the involvement of both ions in auxin metabolism and tranport.

Micro Elements mg/l µM
CoCl2.6H2O 0.025 0.11
CuSO4.5H2O 0.025 0.10
FeNaEDTA 36.70 100.00
H3BO3 6.20 100.27
MnSO4.H20 0.85 5.00
Na2MoO4.2H2O 0.25 1.03
ZnSO4.7H2O 8.60 29.91
Macro Elements mg/l mM
Ca(NO3)2 492.30 2.99
KH2PO4 170.00 1.25
KNO3 1900.00 18.79
MgSO4 180.54 1.50
NH4NO3 1650.00 20.61

Chée, R., and Pool, R.M., Improved Inorganic Media Constituents for In Vitro Shoot Multiplication of Vitis, Scientia Horticulturae, 32 (1987) 85-95.

Filename Language File size
C0248_GB.pdf English 150 KB
C0248_NL.pdf Nederlands 153 KB
C0248_DE.pdf Deutsch 154 KB
C0248_FR.pdf Français 152 KB
C0248_ES.pdf Español 153 KB
C0248_IT.pdf Italiano 151 KB
  • C0248.0010
  • 10 l
  • € 8.50
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