CHÉE & POOL medium including vitamins
In the medium defined by Chée and Pool the original Murashige and Skoog concentration of Chlorine, Iodine and Manganese is decreased, resulting in an improved shoot multiplication of Vitis. Substituting calcium nitrate for calcium chloride improved the quality of grapevine shoots produced in culture. Shoot multiplication was dramatically improved by ommitting Iodine and lowering the concen-tration of Manganese. This might be the result of the involvement of both ions in auxin metabolism and tranport.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.025 | 0.11 |
| CuSO4.5H2O | 0.025 | 0.10 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 6.20 | 100.27 |
| MnSO4.H20 | 0.85 | 5.00 |
| Na2MoO4.2H2O | 0.25 | 1.03 |
| ZnSO4.7H2O | 8.60 | 29.91 |
| Macro Elements | mg/l | mM |
|---|---|---|
| Ca(NO3)2 | 492.30 | 2.99 |
| KH2PO4 | 170.00 | 1.25 |
| KNO3 | 1900.00 | 18.79 |
| MgSO4 | 180.54 | 1.50 |
| NH4NO3 | 1650.00 | 20.61 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 10.00 | 55.5 |
| Nicotinic Acid | 1.00 | 8.12 |
| Pyridoxine HCl | 1.00 | 5.00 |
| Thiamine HCl | 1.00 | 3.00 |
Total concentration Micro and Macro elements including -vitamins: 4458.49 mg/l
Chée, R., and Pool, R.M., Improved Inorganic Media Constituents for In Vitro Shoot Multiplication of Vitis, Scientia Horticulturae, 32 (1987) 85-95.
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water