D0205

De Greef & Jacobs medium

Callus derived from leaf pieces of sugarbeet, exposed to a cold period of 3-9 weeks, could be regenerated into a normal plant after being returned to normal temperature. On media free of growthregulators, a regenerating callus could be -formed with a high regeneration capacity.

Micro Elements mg/l µM
CoCl2.6H2O 0.0025 0.01
CuSO4.5H2O 0.0025 0.01
FeNaEDTA 36.70 100.00
H3BO3 10.62 171.76
KI 1.58 9.54
MnSO4.H2O 1.68 9.94
Na2MoO4.2H2O 0.0025 0.01
ZnSO4.7H2O 1.06 3.69
Macro Elements mg/l mM
CaCl2 226.50 2.04
KCl 600.00 8.05
KNO3 2000.00 19.78
MgSO4 244.33 2.03
NaH2PO4 250.00 2.08
(NH4)2SO4 400.00 3.03

De Greef W. and Jacobs M. In vitro culture of the sugarbeet: Description of a cell line with high regeneration capacity., Plant Science Letters., 17, 55-61 (1979).

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water
Filename Language File size
D0205_GB.pdf English 158 KB
D0205_NL.pdf Nederlands 162 KB
D0205_DE.pdf Deutsch 164 KB
D0205_FR.pdf Français 165 KB
D0205_ES.pdf Español 165 KB
D0205_IT.pdf Italiano 162 KB
  • D0205.0005
  • 5 l
  • € 5.40
  • Order
  • D0205.0050
  • 50 l
  • € 28.60
  • Order
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