D0246

DKW/Juglans medium

DKW medium has been defined for in vitro propagation of Paradox Walnut Rootstock (Juglans hindsii x J. regia) via nodal explants. The explants were placed on medium without growth-regulators for one week and subsequentely on medium containing 6-BAP and IBA. Opti-mum shoot development was supported under 4.5 µM 6-BAP and 5 nM IBA. The basal ends of the tissue culture derived shoots were dipped in 5 mM IBA solution and subsequen-tly rooted within 10 to 14 days in the greenhouse.

Micro Elements mg/l µM
CuSO4.5H2O 0.25 1.00
FeNaEDTA 44.63 121.61
H3BO3 4.80 77.63
MnSO4.H2O 33.80 200.00
Na2MoO4.2H2O 0.39 1.61
ZnSO4.7H2O 17.00 72.19
Macro Elements mg/l mM
CaCl2 112.50 1.01
Ca(NO3)2.2H2O 1664.64 8.30
KH2PO4 265.00 1.95
K2SO4 1559.00 8.95
MgSO4 361.49 3.00
NH4NO3 1416.00 17.70

Total concentration Micro and Macro elements: 5479.50 mg/l

river, J.A., Kuniyuki, A.H. In Vitro Propagation of Paradox walnut Rootstock, Hort. Science, 19(4), August 1984.

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water
Filename Language File size
D0246_GB.pdf English 155 KB
D0246_NL.pdf Nederlands 158 KB
D0246_DE.pdf Deutsch 161 KB
D0246_FR.pdf Français 161 KB
D0246_ES.pdf Español 161 KB
D0246_IT.pdf Italiano 159 KB
Filename Batch number Expiry date Language
D0246_P21737_GB.pdf P21737 05-2025 English
D0246_P21205_GB.pdf P21205 01-2025 English
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