DKW/Juglans medium including vitamins
DKW medium has been defined for in vitro propagation of Paradox Walnut Rootstock (Juglans hindsii x J. regia) via nodal explants. The explants were placed on medium without growth-regulators for one week and subsequentely on medium containing 6-BAP and IBA. Opti-mum shoot development was supported under 4.5 µM 6-BAP and 5 nM IBA. The basal ends of the tissue culture derived shoots were dipped in 5 mM IBA solution and subsequen-tly rooted within 10 to 14 days in the greenhouse.
| Micro Elements | mg/l | µM |
|---|---|---|
| CuSO4.5H2O | 0.25 | 1.00 |
| FeNaEDTA | 44.63 | 121.61 |
| H3BO3 | 4.80 | 77.63 |
| MnSO4.H2O | 33.80 | 200.00 |
| Na2MoO4.2H2O | 0.39 | 1.61 |
| ZnSO4.7H2O | 17.00 | 72.19 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 112.50 | 1.01 |
| Ca(NO3)2.2H2O | 1664.64 | 8.30 |
| KH2PO4 | 265.00 | 1.95 |
| K2SO4 | 1559.00 | 8.95 |
| MgSO4 | 361.49 | 3.00 |
| NH4NO3 | 1416.00 | 17.70 |
Total concentration Micro and Macro elements: 5479.50 mg/l
| Vitamins | mg/l | µM |
|---|---|---|
| Glycine | 2.00 | 26.64 |
| myo-Inositol | 100.00 | 554.94 |
| Nicotinic acid | 1.00 | 8.12 |
| Thiamine HCl | 2.00 | 5.93 |
Total concentration Micro and Macro elements including -vitamins: 5584.50mg/l
river, J.A., Kuniyuki, A.H. In Vitro Propagation of Paradox walnut Rootstock, Hort. Science, 19(4), August 1984.
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water