E0208
Eriksson (ER) medium including vitamins
Eriksson medium was developed for cell suspension cultures of Haplopappus gracilis.
An increase in the growth of cell suspensions, especially with small inocula, was achieved by a 10% reduction of the concentration MS microelements, except for Fe and Zn. Equimolar replacement of ZnSO4.-7H2O by ZnNa2EDTA improved cell growth as well. MnEDTA and CoEDTA did not improve the growth of the cell culture. A reduction of Murashige and Skoog NH4NO3 concentration of 1650 to 1200 mg/l and an increase in phosphate to 2.5 mM also stimulated cell growth.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.0025 | 0.01 |
| CuSO4.5H2O | 0.0025 | 0.01 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 0.63 | 10.19 |
| MnSO4.H2O | 1.69 | 10.00 |
| Na2MoO4.2H2O | 0.025 | 0.10 |
| ZnSO4.7H2O | 9.15 | 31.80 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 332.02 | 2.99 |
| KH2PO4 | 340.00 | 2.50 |
| KNO3 | 1900.00 | 18.79 |
| MgSO4 | 180.54 | 1.50 |
| NH4NO3 | 1200.00 | 14.99 |
| Vitamins | mg/l | µM |
|---|---|---|
| Glycine | 2.00 | 26.64 |
| Nicotinic acid | 0.50 | 4.06 |
| Pyridoxine HCl | 0.50 | 2.43 |
| Thiamine HCl | 0.50 | 1.48 |
Total concentration Micro and Macro elements including -vitamins: 4004.42 mg/l
Eriksson T., Physiol. Plant, 18, 976 (1965).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water