G-418 disulphate

CAS number 108321-42-2
Molecular weight C20H40N4O10.2H2SO4 = 692.7
Assay :> 650 microg / mg

G-418 is an aminoglycoside antibioticum and is applied as a selective agent in transformation experiments. The antibioticum binds to the 30S subunit of the prokaryotic ribosome, thereby inhibiting protein synthesis as well as generating errors in the transcription of the genetic code. Ribosomes of mitochondria and chloroplasts of higher plants are related to bacterial ribosomes and are also susceptible to aminoglycosides. Being a derivative of gentamycin, the antibioticum contains a additional 3’OH that can be phosphorylated by NPT II. As a result of this phosporylation, the charge and the steriometric conformation of the G-418 molecule changes in such a way that the antibioticum is no longer capable of binding to the specific ribosome binding sites.G-418 is used as an alternative for kanamycin in monocots, e.g., rice, Lolium, Graminea which are highly resistant to the latter. In all cases G-418 was shown to be more effective. This is most probably due to the better binding characteristics of the gentamycine shaped structure of G-418.

Storage condition

  • Store at 2-8 °C
  • Soluble in water

Hazard statements

  • H312 Harmful in contact with skin
  • H332 Harmful if inhaled
  • H360 May damage fertility or the unborn child

Precautionary statements

  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P302 IF ON SKIN:
  • P304 IF INHALED:
  • P308 IF exposed or concerned:
  • P313 Get medical advice/attention
  • P341 If breathing is difficult
  • P352 Wash with soap and water
Filename Language File size
G0175_GB.pdf English 167 KB
G0175_NL.pdf Nederlands 171 KB
G0175_DE.pdf Deutsch 176 KB
G0175_FR.pdf Français 174 KB
G0175_ES.pdf Español 173 KB
G0175_IT.pdf Italiano 171 KB
Filename Batch number Expiry date Language
G0175_015716_GB.pdf 015716 09-2023 English
G0175_015099_GB.pdf 015099 12-2022 English
G0175_013800_GB.pdf 013800 05-2021 Expired English
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  • 1 g
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  • 5 g
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