G0210

Gamborg B5 medium including vitamins

B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l. B5 medium has been defined for the growth of cell suspensions of soybean root cells in the presence of 2,4 D. Nitrate was required in a concentration of 20-30 mM. An addition of 2 mM ammoniumsulphate led to an increase in cellgrowth. NH4+ when added as the sole source of nitrogen, did not support growth. Similar results were obtai-ned when NH4NO3 was substi-tuted for (NH4)2SO4. However, ammonium ions depressed growth when the concen-tration excee-ded 2 mM. Variati-ons in the concentrations of phosphate, calcium and magnesi-um resulted in relatively minor chan-ges in growth rate. Thiamine is known to be an essenti-al nutrient for cell growth and is increa-sed in concentrati-on up to 10 mg/l.

Micro Elements mg/l µM
CoCl2.6H2O 0.025 0.11
CuSO4.5H2O 0.025 0.10
FeNaEDTA 36.70 100.00
H3BO3 3.00 48.52
KI 0.75 4.52
MnSO4.H2O 10.00 59.16
Na2MoO4.2H2O 0.25 1.03
ZnSO4.7H2O 2.00 6.96
Macro Elements mg/l mM
CaCl2 113.23 1.02
KNO3 2500.00 24.73
MgSO4 121.56 1.01
NaH2PO4 130.44 1.09
(NH4)2SO4 134.00 1.01
Vitamins mg/l µM
myo-Inositol 100.00 554.94
Nicotinic acid 1.00 8.12
Pyridoxine HCl 1.00 4.86
Thiamine HCl 10.00 29.65

Total concentration Micro and Macro elements including -vitamins: 3163.98 mg/l

Gamborg O.L., Miller R.A., Ojima K., Nutrient requirement of suspensions cultures of soybean root cells. Exp. Cell Res., 50, 151 (1968).

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water
Filename Language File size
G0210_GB.pdf English 157 KB
G0210_NL.pdf Nederlands 161 KB
G0210_DE.pdf Deutsch 163 KB
G0210_FR.pdf Français 163 KB
G0210_ES.pdf Español 162 KB
G0210_IT.pdf Italiano 160 KB
Filename Batch number Expiry date Language
G0210_P21174_GB.pdf P21174 09-2026 English
G0210_P22175_GB.pdf P22175 09-2026 English
G0210_P21847_GB.pdf P21847 06-2026 English
G0210_P21219_GB.pdf P21219 02-2026 English
G0210_P21030_GB.pdf P21030 12-2025 English
G0210_P21029_GB.pdf P21029 12-2025 English
G0210_P20608_GB.pdf P20608 09-2025 English
G0210_P20609_GB.pdf P20609 09-2025 English
G0210_P20606_GB.pdf P20606 09-2025 English
G0210_P20378_GB.pdf P20378 07-2025 English
G0210_P20379_GB.pdf P20379 07-2025 English
G0210_P19510_GB.pdf P19510 01-2025 English
G0210_P19069_GB.pdf P19069 10-2024 English
G0210_P19022_GB.pdf P19022 08-2024 English
G0210_P18684_GB.pdf P18684 06-2024 English
G0210_P18079_GB.pdf P18079 01-2024 Expired English
G0210_P18080_GB.pdf P18080 01-2024 Expired English
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