KBZ Medium

Bacterial speck of tomatoes is caused by the bacterium Pseudomonas syringae pv. tomato (Pst). The bacterium can be introduced by the use of Pst-contaminated seeds. Therefore, detection of Pst in seeds of tomato is common. For the detection of Pst, seeds are first soaked in buffer. Then a stomacher is used for the release of bacteria from the seeds. The bacteria are concentrated by centrifugation. Then dilution plating on two semi-selectice media KBZ and KBBC is performed. Suspected colonies are transferred to KB and finally identified by PCR or a pathogenicity assay. Pst forms small, flat and pink-colored colonies on KBZ after ca. 5 days. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Filename Language File size
K5129_GB.pdf English 138 KB
K5129_NL.pdf Nederlands 142 KB
K5129_DE.pdf Deutsch 146 KB
K5129_FR.pdf Français 143 KB
K5129_ES.pdf Español 143 KB
K5129_IT.pdf Italiano 141 KB
  • K5129.1000
  • 1 kg
  • € 170.70
  • Order
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