L0218
Litvay medium including vitamins
Litvay’s medium is composed for the in vitro culture of cell suspension of Daucus carotus and finally for Pinus taeda L. An increase of Increasing the magnesium concentration from 0.75 mM to 7.5 mM and decreasing the calcium concentration from 1.5 mM to 0.15 mM was also of positive -influence. However, these alterations are not as drastical as the improvement by the enrichement of the medium by additio-nal -phosphate.the phosphate concentration from 0.5 mM to 2.5 mM was essential for improved cell growth and embryo-genesis.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.125 | 0.53 |
| CuSO4.5H2O | 0.50 | 2.00 |
| FeNaEDTA | 36.70 | 100.00 |
| H3BO3 | 31.00 | 501.37 |
| KI | 4.15 | 25.00 |
| MnSO4.H2O | 21.00 | 124.25 |
| Na2MoO4.2H2O | 1.25 | 5.17 |
| ZnSO4.7H2O | 43.00 | 149.54 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 16.61 | 0.15 |
| KH2PO4 | 340.00 | 2.50 |
| KNO3 | 1900.00 | 18.79 |
| MgSO4 | 903.38 | 7.51 |
| NH4NO3 | 1650.00 | 20.61 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 100.00 | 554.94 |
| Nicotinic acid | 0.50 | 4.06 |
| Pyridoxine HCl | 0.10 | 0.49 |
| Thiamine HCl | 0.10 | 0.30 |
Total concentration Micro and Macro elements including vitamins: 5048.42 mg/l
Litvay J.D., Verma D.C., Morris A.J., Plant Cell Rep., 4, 325 (1985).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P273 Avoid release to the environment
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water