M0222

Murashige & Skoog medium including vitamins

Original concentration, (1962)

MS medium is the most used tissue culture medium, of which many variations have been developed. The medium is originated from White’s medium and originally developed for the cultivation of Nicotiana tabacum calli. Compared to White medium, the concentration of all ingredients is increased. An increase to 50-60 mM nitrogen stimulated the growth of Nicotiana cells significantly, however a concentration of 80 mM and higher was clearly disadvantageous to the cells. The increase of all other elements, especially the macro -elements, also stimulated the growth of the calli. Due to the high concentration of minerals, MS medium is a very rich and -saline -medium and may be too salty to certain plant species. To avoid this problem, MS is often used with the micro elements in full concentration, but with the macro elements in respectively half or three-quarter of the concentration as originally described by the authors. Sometimes the original MS vitamines are replaced by the vitamins of Linsmaier and Gamborg B5 medium regarding the higher concentration of Thiamine in relation to the requirement of this vitamin by plants.

Micro Elements mg/l µM
CoCl2.6H2O 0.025 0.11
CuSO4.5H2O 0.025 0.10
FeNaEDTA 36.70 100.00
H3BO3 6.20 100.27
KI 0.83 5.00
MnSO4.H2O 16.90 100.00
Na2MoO4.2H2O 0.25 1.03
ZnSO4.7H2O 8.60 29.91
Macro Elements mg/l mM
CaCl2 332.02 2.99
KH2PO4 170.00 1.25
KNO3 1900.00 18.79
MgSO4 180.54 1.50
NH4NO3 1650.00 20.61
Vitamins mg/l µM
Glycine 2.00 26.64
myo-Inositol 100.00 554.94
Nicotinic acid 0.50 4.06
Pyridoxine HCl 0.50 2.43
Thiamine HCl 0.10 0.30

Total concentration Micro and Macro elements including vitamins: 4405.19 mg/l

Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water
Filename Language File size
M0222_GB.pdf English 628 KB
M0222_NL.pdf Nederlands 639 KB
M0222_DE.pdf Deutsch 630 KB
M0222_FR.pdf Français 653 KB
M0222_ES.pdf Español 653 KB
M0222_IT.pdf Italiano 646 KB
M0222_PL.pdf Polish 657 KB
M0222_KO.pdf Korean 767 KB
M0222_DA.pdf Danish 629 KB
Filename Batch number Expiry date Language
M0222_P22791_GB.pdf P22791 02-2027 English
M0222_P22776_GB.pdf P22776 02-2027 English
M0222_P22668_GB.pdf P22668 01-2027 English
M0222_P22787_GB.pdf P22787 01-2027 English
M0222_P22139_GB.pdf P22139 09-2026 English
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M0222_P21992_GB.pdf P21992 07-2026 English
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M0222_P21443_GB.pdf P21443 03-2026 English
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M0222_P21138_GB.pdf P21138 12-2025 English
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M0222_P20258_GB.pdf P20258 05-2025 English
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M0222_P19707_GB.pdf P19707 02-2025 English
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M0222_P19559_GB.pdf P19559 01-2025 English
M0222_P19481_GB.pdf P19481 12-2024 English
M0222_P19297_GB.pdf P19297 11-2024 English
M0222_P19318_GB.pdf P19318 11-2024 English
M0222_P19307_GB.pdf P19307 11-2024 English
M0222_P19291_GB.pdf P19291 11-2024 English
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M0222_P19216_GB.pdf P19216 10-2024 English
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M0222_P19168_GB.pdf P19168 09-2024 English
M0222_P18890_GB.pdf P18890 08-2024 English
M0222_P18742_GB.pdf P18742 07-2024 English
M0222_P18719_GB.pdf P18719 07-2024 English
M0222_P18720_GB.pdf P18720 07-2024 English
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M0222_P18617_GB.pdf P18617 06-2024 English
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M0222_P18643_GB.pdf P18643 06-2024 English
M0222_P18644_GB.pdf P18644 06-2024 English
M0222_P18625_GB.pdf P18625 06-2024 English
M0222_P18553_GB.pdf P18553 05-2024 English
M0222_P18388_GB.pdf P18388 04-2024 English
M0222_P18338_GB.pdf P18338 03-2024 Expired English
M0222_P18171_GB.pdf P18171 03-2024 Expired English
M0222_P18357_GB.pdf P18357 03-2024 Expired English
M0222_P17910_GB.pdf P17910 12-2023 Expired English
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