M5133

MT Medium

The MT (Milk-Tween) Medium is a semi-selective medium for the detection of Pseudomonas syringae pv. syringae (Pss), Pseudomonas savastanoi pv. phaseolicola (Pspha) and Xanthomonas axonopodis pv. phaseoli (Xap) in bean seed. The medium relies on the ability of the micro-organisms to hydrolyze casein. Suspected isolates are transferred to YDC (Xap) or KB (Pss and Pspha). Finally, the identity of suspected col-onies is determined by PCR or a pathogenicity test.The colonies of Pspha and Pss are cream white, flat circular, 4-5 mm in diameter and produce a blue fluorescent pigment under UV light. Xap colonies (3 – 3.5 mm in diameter) are yellow, non fluorescent and typical two zones surround colonies: a bigger, clear zone of casein hydrolysis and a smaller zone of Tween 80 lipolysis. Xap var. fuscans (1 – 2 mm in diameter) produces a brown pigment within 5 days. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Filename Language File size
M5133_GB.pdf English 140 KB
M5133_NL.pdf Nederlands 145 KB
M5133_DE.pdf Deutsch 149 KB
M5133_FR.pdf Français 146 KB
M5133_ES.pdf Español 146 KB
M5133_IT.pdf Italiano 144 KB
Filename Batch number Expiry date Language
M5133_P13044_GB.pdf P13044 09-2018 Expired English
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  • M5133.1000
  • 1 kg
  • € 66.00
  • Order
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