M5167

MSP Medium

MSP (Modified Sucrose Peptone) medium is a suitable medium for the detection of Pseudomonas savastanoi pv. phaseolicola (Pspha) and Pseudomonas syringae pv. syringae (Pss). Addition of bromothymol blue gives this medium a blue appearance. The color of bacterial colonies is influenced by this compound. The assay starts with dilution plating of bacterial extract from seeds on MSP. Then suspected colonies from MSP can be transferred to King’s B Medium (K5165). Finally, the identity of suspected isolates is confirmed by a pathogenicity test or PCR. Colonies of Pspha and Pss are ca. 3 mm in diameter, circular, raised, globose, glistening and light yellow with a denser centerThe medium around Pspha colonies turns light yellow after three days of incubation. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Filename Language File size
M5167_GB.pdf English 140 KB
M5167_NL.pdf Nederlands 144 KB
M5167_DE.pdf Deutsch 148 KB
M5167_FR.pdf Français 145 KB
M5167_ES.pdf Español 145 KB
M5167_IT.pdf Italiano 143 KB
Filename Batch number Expiry date Language
M5167_P14605_GB.pdf P14605 01-2020 English
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  • M5167.1000
  • 1 kg
  • € 71.70
  • Order
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