N0252

NLN medium

The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg/l. To prepare the proper NLN medium formulation 500 mg/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.The composition of the NLN medium originated from the medium described by Nitsch. The medium was developed for anthercultures of Brassica Napus in liquid medium and the induction of haploid plants from isolated pollen. NLN medium is provided free of Calcium nitrate. In the original medium Ca(NO3)2.4H2O is present in a concentration of 500 mg/l. To prepare the proper NLN medium formulation 500 mg/l Ca(NO3)2.4H2O has to be added extra to the already dissolved powdered medium.

Micro Elements mg/l µM
CoCl2.6H2O 0.025 0.11
CuSO4.5H2O 0.025 0.10
FeNaEDTA 36.70 100.00
H3BO3 10.00 161.73
MnSO4.H2O 18.95 111.94
Na2MoO4.2H2O 0.25 1.03
ZnSO4.7H2O 10.00 34.78
CoCl2.6H2O 0.025 0.11
CuSO4.5H2O 0.025 0.10
FeNaEDTA 36.70 100.00
H3BO3 10.00 161.73
MnSO4.H2O 18.95 111.94
Na2MoO4.2H2O 0.25 1.03
ZnSO4.7H2O 10.00 34.78
Macro Elements mg/l mM
Ca(NO3)2.4H2O Ommitted (500 mg/l)
KH2PO4 125.00 0.92
KNO3 125.00 1.24
MgSO4 61.00 0.51
Ca(NO3)2.4H2O Ommitted (500 mg/l)
KH2PO4 125.00 0.92
KNO3 125.00 1.24
MgSO4 61.00 0.51

Total concentration Micro and Macro elements: 386.95 mg/l

Total concentration Micro and Macro elements: 386.95 mg/l

Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982

Lichter, R., Z. Planzephysiol., 103, 229-237, 1981, Lichter, R., Z. Planzephysiol., 105, 427-434, 1982

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation
  • H412 Harmful to aquatic life with long lasting effects

Precautionary statements

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P273 Avoid release to the environment
  • P302 IF ON SKIN:
  • P305 IF IN EYES:
  • P351 Rinse continuously with water for several minutes
  • P352 Wash with soap and water
Filename Language File size
N0252_GB.pdf English 157 KB
N0252_NL.pdf Nederlands 161 KB
N0252_DE.pdf Deutsch 163 KB
N0252_FR.pdf Français 163 KB
N0252_ES.pdf Español 163 KB
N0252_IT.pdf Italiano 161 KB
  • N0252.0010
  • 10 l
  • € 9.20
  • Order
  • N0252.0050
  • 50 l
  • € 28.60
  • Order
* All prices are ex-works Haarlem NL. Salestax, local prices and availability may vary in your region/country. Please contact our sales department or read our terms of sales.