S0225
Schenk & Hildebrandt Basal salt medium
Schenk en Hildebrandt medium has been developed for growth of both monocotyle and dicotyle cell suspensions. A high level of auxin-type growth regulators, 2,4-D (0.5 mg/l) and 4-CPA (2.0 mg/l), g-enerally favoured monocoty-ledonous cell cul-tu-r-es, while low levels of cytokin, kinetin (0.1 mg/l), were essential for most dicotyledonous cell cultures.
| Micro Elements | mg/l | µM |
|---|---|---|
| CoCl2.6H2O | 0.10 | |
| CuSO4.5H2O | 0.20 | |
| FeNaEDTA | 19.80 | |
| H3BO3 | 5.00 | |
| KI | 1.00 | |
| MnSO4.H2O | 10.00 | |
| Na2MoO4.2H2O | 0.10 | |
| ZnSO4.7H2O | 1.00 |
| Macro Elements | mg/l | mM |
|---|---|---|
| CaCl2 | 151.00 | |
| KNO3 | 2500.00 | |
| MgSO4 | 195.05 | |
| (NH4)H2PO4 | 300.00 |
| Vitamins | mg/l | µM |
|---|---|---|
| myo-Inositol | 1000.0 | |
| Nicotinic acid | 5.0 | |
| Pyridoxine HCl | 0.5 | |
| Thiamine HCl | 5.0 |
Total concentration Micro and Macro elements including vitamins: 4193.75mg/l
Schenk R.U. and Hildebrandt A.C., Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50, 199 (1972).
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P302 IF ON SKIN:
- P305 IF IN EYES:
- P351 Rinse continuously with water for several minutes
- P352 Wash with soap and water