SCM Medium

Bacterial canker is the most important bacterial disease of tomato. The causal organism is Clavibacter michiganensis subsp. michiganensis (Cmm) and this bacterium can be intro-duced by contaminated seeds. For the detection of Cmm, toma-to seeds are first soaked in buffer. Then a stomacher is used for the release of bacteria from the seeds. After the concentra-tion of the bacteria, dilution plating on two semi-selective media is performed. SCM medium is such a semi-selective media. Actually, there are several modifications in use con-cerning the used carbon source, LiCl and the addition of antibi-otics. This medium is used in combination with D2ANX medi-um (D5128). After dilution plating suspected isolates are trans-ferred to YDC. Finally the identity of suspected isolates is determined by a pathogenicity test or PCR. The colonies of Clavibacter michiganensis subsp. michiganensis on SCM are small, light to dark grey, glistening, fluidal and often irregularly shaped. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Filename Language File size
S5127_GB.pdf English 143 KB
S5127_NL.pdf Nederlands 146 KB
S5127_DE.pdf Deutsch 149 KB
S5127_FR.pdf Français 147 KB
S5127_ES.pdf Español 147 KB
S5127_IT.pdf Italiano 147 KB
  • S5127.1000
  • 1 kg
  • € 95.40
  • Order
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