C0204

CHU (N6) Medium including vitamins

Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.Chu (N6) medium is defined to improve the formation, growth and differentiation of pollen callus in rice. The concentration of ammonium proved to be crucial for the development of callus. The optimum -concentration NH4+ is 7.0 mM (equal to 3.5 mM (NH4)2SO4). Higher concentrations of ammonium drastically inhibited the growth and -differentiation of the rice pollen. The concentration of KNO3 and the other medi-um compo-nents did not affect the development of the -callus.

Micro Elements mg/l µM
FeNaEDTA 36.70 100.00
H3BO3 1.60 25.88
KI 0.80 4.81
MnSO4.H2O 3.33 19.70
ZnSO4.7H2O 1.50 5.22
Macro Elements mg/l mM
CaCl2 125.33 1.13
KH2PO4 400.00 2.94
KNO3 2830.00 27.99
MgSO4 90.27 0.75
(NH4)2SO4 463.00 3.50
Vitamins mg/l µM
Glycine 2.00 26.64
Thiamine HCl 1.00 2.96
Pyridoxine HCl 0.50 2.43
Nicotinic acid 0.50 4.06

Total concentration Micro and Macro elements including -vitamins: 3956.53 mg/l

Chu C.C, The N6 medium and its application to anther culture of -cereal crops, Proc. Symp. Plant Tissue Cult., Peking, 43 (1978).Chu C.C. et al., Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen -sources. Scienta Sinic., 18, 659 (1975).

Filename Language File size
C0204_GB.pdf English 150 KB
C0204_NL.pdf Nederlands 151 KB
C0204_DE.pdf Deutsch 154 KB
C0204_FR.pdf Français 150 KB
C0204_ES.pdf Español 150 KB
C0204_IT.pdf Italiano 149 KB
Filename Batch number Expiry date Language
C0204_P12925_GB.pdf P12925 08-2018 English
C0204_P12939_GB.pdf P12939 08-2018 English
C0204_P12484_GB.pdf P12484 03-2018 Expired English
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