C0228

CLC / Ipomoea CP medium

Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM. Sweetpotato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 µM 2,4-D and 1 µM 6-BAP. Increa-sing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embry-ogenic callus was reduced by 40%. The development of embryos, trigge-red by the removal of 2,4-D and 6-BAP, was enhan-ced by decrea-sing ammonium (NH4+) from 20 to 10 mM.

Micro Elements mg/l µM
CoCl2.6H2O 0.025
CuSO4.5H2O 0.025
FeNaEDTA 36.70
H3BO3 6.20
KI 0.83
MnSO4.H20 16.90
Na2MoO4.2H2O 0.25
ZnSO4.7H2O 8.60
Macro Elements mg/l mM
CaCl2 332.02
KCl 2237.00
KH2PO4 170.00
KNO3 2022.00
MgSO4 180.54
NH4NO3 1601.00
Vitamins mg/l µM
myo-Inositol 90.10
Nicotinic acid 1.23
Pyridoxine HCl 1.03
Thiamine HCl 1.69

Total concentration Micro and Macro elements (CP) including -vitamins: 6706.14 mg/l

Cheé R. et al., Optimizing Embryogenic Callus and Embryo Growth of a synthetic seed system for Sweetpotato by varying media nutrient concentrations. J. Am. Soc. Hort. Sci. 117, 663 (1992).

Filename Language File size
C0228_GB.pdf English 152 KB
C0228_NL.pdf Nederlands 153 KB
C0228_DE.pdf Deutsch 154 KB
C0228_FR.pdf Français 153 KB
C0228_ES.pdf Español 153 KB
C0228_IT.pdf Italiano 152 KB
Filename Batch number Expiry date Language
C0228_P11656_GB.pdf P11656 05-2018 English
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