CAS number 9062-07-1

D2ANX is a semi-selective medium, which is used to detect Clavibacter michiganensis subsp. michiganensis (Cmm). This medium, with a relatively low is often used in combination with the more selective mSCM medium (S5127). Despite the slow growth of Cmm colonies the evaluation of plates can already be performed after 6-7 days of incubation. On mSCM, the growth is more slow and Cmm colonies can only be seen after about 9-10 days. On D2ANX, Cmm colonies are glistening, yellow and mucoid. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

CAS number 67-68-5
Molecular weight C₂H₆OS = 78.1
Assay > 99 %
H2O < 0.1 %

Eriksson T., Physiol. Plant, 18, 976 (1965).

Eriksson T., Physiol. Plant, 18, 976 (1965).

Gamborg O.L., Miller R.A., Ojima K., Nutrient requirement of suspensions cultures of soybean root cells. Exp. Cell Res., 50, 151 (1968).

Gamborg O.L., Miller R.A., Ojima K., Nutrient requirement of suspensions cultures of soybean root cells. Exp. Cell Res., 50, 151 (1968).

CAS number 9000-07-1

CAS number 71010-52-1
Loss on drying < 15 %
Gel strength 400 - 700 g/cm2

Pseudomonas syringae pv. syringae (Pss) is the causal organism of bacterial brown spot of beans. This bacterium is seed borne and therefore its detection on seeds is important. KBBC medium is a rather selective medium to detect Pss on seeds of beans. This medium is based on King’s B Medium (K5165), however in KBBC Medium boric acid (1.5 g/liter), cephalexin and nystatin are added. Nystatin is used to control fungi. As an alternative, cycloheximide, a more potent fungicide, can be used. KBBC is much more selective than MSP (M5167) and in general the recovery of Pss is smaller on KBBC than on MSP. Pspha, unlike Pss, will not grow on KBBC. Therefore, the chance of detection of Pss is higher when both complementary media are used.Detection of Pss is performed by the dilution plating of bacterial extract on KBBC and MSP. Then Pss-suspected isolates are transferred to KB medium. Finally, the identification of suspected colonies can be performed by a pathogenicity assay or PCR.Colonies of Pss on KBBC are 3-4 mm in diameter, flat, circular, translucent, creamy white and show blue fluorescence under UV light. This medium can also be used for the detection of seed borne Ps porri, Ps pisi and Ps tomato on seed of resp. leek, pea and tomato. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Bacterial speck of tomatoes is caused by the bacterium Pseudomonas syringae pv. tomato (Pst). The bacterium can be introduced by the use of Pst-contaminated seeds. Therefore, detection of Pst in seeds of tomato is common. For the detection of Pst, seeds are first soaked in buffer. Then a stomacher is used for the release of bacteria from the seeds. The bacteria are concentrated by centrifugation. Then dilution plating on two semi-selectice media KBZ and KBBC is performed. Suspected colonies are transferred to KB and finally identified by PCR or a pathogenicity assay. Pst forms small, flat and pink-colored colonies on KBZ after ca. 5 days. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Linsmaier E.M. and Skoog F., Physiol. Plantarum, 18, 100, (1965).

Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

MSP (Modified Sucrose Peptone) medium is a suitable medium for the detection of Pseudomonas savastanoi pv. phaseolicola (Pspha) and Pseudomonas syringae pv. syringae (Pss). Addition of bromothymol blue gives this medium a blue appearance. The color of bacterial colonies is influenced by this compound. The assay starts with dilution plating of bacterial extract from seeds on MSP. Then suspected colonies from MSP can be transferred to King’s B Medium (K5165). Finally, the identity of suspected isolates is confirmed by a pathogenicity test or PCR. Colonies of Pspha and Pss are ca. 3 mm in diameter, circular, raised, globose, glistening and light yellow with a denser centerThe medium around Pspha colonies turns light yellow after three days of incubation. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

The MT (Milk-Tween) Medium is a semi-selective medium for the detection of Pseudomonas syringae pv. syringae (Pss), Pseudomonas savastanoi pv. phaseolicola (Pspha) and Xanthomonas axonopodis pv. phaseoli (Xap) in bean seed. The medium relies on the ability of the micro-organisms to hydrolyze casein. Suspected isolates are transferred to YDC (Xap) or KB (Pss and Pspha). Finally, the identity of suspected col-onies is determined by PCR or a pathogenicity test.The colonies of Pspha and Pss are cream white, flat circular, 4-5 mm in diameter and produce a blue fluorescent pigment under UV light. Xap colonies (3 – 3.5 mm in diameter) are yellow, non fluorescent and typical two zones surround colonies: a bigger, clear zone of casein hydrolysis and a smaller zone of Tween 80 lipolysis. Xap var. fuscans (1 – 2 mm in diameter) produces a brown pigment within 5 days. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Bacterial spot is an important bacterial disease of peppers. Two different bacteria, Xanthomonas campestris pv. vesicatoria (Xcv) and Xanthomonas vesicatoria (Xv) can incite this seed borne disease. mTMB (modified Tween Medium B) is a semi-selective medium for detection of Xcv and Xv on seeds of pepper and tomato. The colonies of Xcv and Xv on mTMB plates are yellow, slightly mucoid, mounded and round. Xcv utilizes Tween 80 and in 3-7 days a white crystalline halo usually forms around the yellow colony. Contaminated seed lots can be detected by dilution plating of the bacterial extract on CKTM, mKM or MXV. Suspected isolates are then transferred to YDC. Finally, the identity of the suspected isolates can be deter-mined by a pathogenicity test or PCR. For detailed information about Duchefa’s Phytopathological media have a look at our specialized brochure about this topic. Brochure can be found at the “Brochure section” at the home page of this website.

Syngonium stage I & II
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Finer & Nagasawa Modification : 1.6 x concentration of KNO₃ & 0,5 x concentration of NH₄NO₃
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

including Modified Vitamins
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

van der Salm Modification (1994)
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

van der Salm Modification (1994)
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Original concentration, (1962)
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

including MES buffer, (500 mg/l)
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

including Nitsch Vitamins
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

including MES buffer, (500 mg/l)
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

including Gamborg B₅ vitamins
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Original concentration, (1962)
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Modification No.1 : 1/2 concentration Macro elements
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Modification No.1 : 1/2 concentration Macro elements
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

modification No. 2 : 3/4 Concentration Macro elements
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

modification No. 2 : 3/4 Concentration Macro elements
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Modification No. 3 : 1/2 Concentration NH₄NO₃ and KNO₃
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Modification No. 3 : 1/2 Concentration NH₄NO₃ and KNO₃
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Modification No. 4 : NH₄NO₃ Free
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Modification No. 5 : NH₄NO₃ replaced by NaNO₃
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

Shoot multiplication Medium B
Murashige T. and Skoog F., Physiol. Plant, 15, 473 (1962).

CAS number 1263-89-4
Molecular weight C₂₃H₄₅N₅O₁₄.xH₂SO₄
Assay > 675 microg / mg

Druart. P., Sci. Hort., 12, 339-342, (1980).,Quoirin M. and Lepoivre P., Acta Hort, 78, 437, (1977).,Valles. M., Boxus, Ph., Acta Hort., 212, (1987).

Druart. P., Sci. Hort., 12, 339-342, (1980).,Quoirin M. and Lepoivre P., Acta Hort, 78, 437, (1977).,Valles. M., Boxus, Ph., Acta Hort., 212, (1987).

Rugini E., In vitro propagation of some olive cultivars, Scientia Horticulturae 24, 123 (1984)., Jacoboni A., Luppino M., Rugini E., Role of basal shoot darkening Scientia Horticolturae, 53:63 (1993)

CAS number 723-46-6
Molecular weight C₁₀H₁₁N₃O₃S = 253.3